Summary

Serum / plasma may be used with this test . When a specimen is added to the test , IgG and IgM antibodies in the specimen sample react with gold particles coated with CMV envelope proteins . As this specimen / particle mixture migrates along the length of the test , the anti - CMV IgG or IgM antibody particle complex is captured by the relevant IgG and / or IgM test bands located in the test device window causing a pale to dark red band to form at the Test region bf the test device window . The intensity of the bands will vary depending upon the amount of antibody present in the sample . The appearance of any color in test region should be considered as positive for IgG and / or IgM antibody . A red procedural control line should always develop in the test device window to indicate that the test has been performed properly .

Specimen collection

For serum or plasma , colect blood into a container without anticoagulant . If the specimen cannot be tested on the day of collection , store the serum or plasma specimen in a refrigerator or freezer . Bring the specimens to room temperature before testing .

Test procedure
Dilute the serum (or plasma)specimen 10 times with diluent(e.g.50ul specimen add 450ul diluent ).
Open a pouch containing a cassette,remove the test kit from the pouch and place it horizontally on the desk.
Pipette 1 or 2 drops of diluted specimen into the sample well of the cassette.
Read results within 10-15 minutes.Do not read results after 20 minutes

Procedure and result analysis